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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene has become observed in many tumors, which results in chromosome instability, and so may well add to tumorigenesis. This protein was discovered to associate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a vital job in marketing cell-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, happen to be described. Two extra splice variants were described but specific nucleotide sequence info isn't nonetheless accessible.

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Blocking peptides are peptides that bind specially towards the focus on antibody and block antibody binding. These peptide ordinarily includes the epitope recognized by the antibody.

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This guidebook outlines additional feasible optimization of cellular and immunoassay parameters to guarantee the very best success are...

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This guide outlines further more doable optimization of mobile and immunoassay parameters to be sure the absolute best success are...

Antibodies bound to the blocking peptide not bind towards the epitope around the target protein. By evaluating the staining within the blocked antibody vs . the antibody by itself, you can see which staining is precise.

Focus on Data Cyclin E belongs for the very conserved cyclin family members, whose associates are characterized by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which add for the temporal coordination of each mitotic party. Cyclin E kinds a fancy with and features to be a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates within the G1-S period boundary and it is degraded as cells development via S section.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...

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